Formulation and Quantitative Phytochemical Analysis of Herbal Tea Blended from Three Indigenous Medicinal Herbs
Research Article
DOI:
https://doi.org/10.21276/pt.2025.v2.i3.11Keywords:
Herbal tea, Quantitative phytochemical analysis, Ocimum gratissimum L., Ocimum tenuiflorum L., Girardinia diversifolia (Link) Friis.Abstract
In recent years, herbal teas have gained popularity among consumers and are prepared from plants other than the Camellia genus. It is widely consumed around the world because it is believed to have health benefits and possess antioxidant potential. Therefore, the present study aimed to formulate an herbal tea blend using three indigenous medicinal plants [Ocimum gratissimum L, Ocimum tenuiflorum L, and Girardinia diversifolia (Link) Friis] by sustainably processing them through solar drying, and to evaluate their quantitative phytochemical characterization and sensory acceptability. Quantitative phytochemical analysis revealed that the 15-minute boiled sample exhibited the highest levels of TPC (147.98± 0.000 mg GAE/g), TFC (8.26±0.000 mg QE/g), and TTC (191.9± 0.0005 mg TAE/g), while the 2-minute sample also retained appreciable levels (TPC: 120.37±0.002 mg GAE/g; TFC: 0.013±0.000 mg QE/g; TTC: 35.5±0.0005 mg TAE/g), highlighting its suitability for instant formulations such as sachets. Additionally, the formulated blend consistently outperformed a commercial herbal tea (Organic India) across all parameters. Sensory evaluation showed favourable consumer responses regarding colour, aroma, taste, and mouthfeel. These findings suggest that both extended and short-duration boiling can yield health-beneficial infusions, supporting the broader application of indigenous herbs in functional beverage development
References
Singh A, Raghuvanshi R. S, Bhatnagar A. Herbal tea formulation using different flavoured herbs with dried corn silk powder and its sensory and phytochemical analysis. Syst Microbiol Biomanuf. 2021; 1(3):336–343. https://doi.org/10.1007/s43393-021-00029-9
Saeed S, Rasoo S, Ishtiaq S, Hassan S, Waheed I, Saeed M A. Phytochemical investigation of irritant constituents of Cuscuta reflex. Int J Agric Biol. 2014; 16(6):1161–1166. http://www.fspublishers.org
Cao Y, Ning Z, Zhao Y. Challenges and progressions in herbal genomics. Fron Plant Sci. 2023; 14:1273769. https://doi.org/10.3389/fpls.2023.1273769
Brendler T, Brinckmann J A, Daoust M, He H, Masé G, Steffan K, Williams M. Suitability of botanical extracts as components of complex mixtures used in herbal tea infusions challenges and opportunities. Front Pharmacol. 2022; 13:1013340. https://doi.org/10.3389/fphar.2022.1013340
Long T, Hu R, Cheng Z, Xu C, Hu Q, Liu Q. Ethnobotanical study on herbal tea drinks in Guangxi. J Ethnobiol Ethnomed. 2023; 19(1):10. https://doi.org/10.1186/s13002-023-00579-3
Ugbogu O C, Emmanuel O, Agi G O, Ibe C, Ekweogu C N, Ude V C, Ugbogu E A. A review on the traditional uses, phytochemistry, and pharmacological activities of clove basil (Ocimum gratissimum L.). Heliyon. 2021; 7(11). https://doi.org/10.1016/j.heliyon.2021.e08404
Priyanka C, Shivika S, Vikas S. Ocimum gratissimum: a review on ethnomedicinal properties, phytochemical constituents, and pharmacological profile. Approaches Med Aromat Plants. 2018; 251–270. https://doi.org/10.1007/978-981-13-0535-1_11
Gunardi D, Sudradjat S E, Timotius KH. Healing capacities of nettles: dendrocnide, girardinia, laportea, and urtica. Phytomed Plus. 2023; 3(2):100438. https://doi.org/10.1016/j.phyplu.2023.100438
Shrestha SS, Sut S, Ferrarese I, Barbon Di Marco S, Zengin G, De Franco M, Rajbhandary S. Himalayan nettle Girardinia diversifolia as a candidate ingredient for pharmaceutical and nutraceutical applications, phytochemical analysis and in vitro bioassays. Molecules. 2020; 25(7):1563. https://doi.org/10.3390/molecules25071563
Pattanayak P, Behera P, Das D, Panda SK, Ocimum sanctum Linn. A reservoir plant for therapeutic applications: An overview. Pharmacogn Rev. 2010; 4(7): 95–105. https://doi.org/10.4103/0973-7847.65323
Jamshidi N, Cohen MM. The clinical efficacy and safety of Tulsi in humans: a systematic review of the literature. Evid Based Complement Alternat Med. 2017; 1: 9217567. https://doi.org/10.1155/2017/9217567
Shahidi F, Hossain A. Bioactives in spices and spice oleoresins Phytochemicals and their beneficial effects in food preservation and health promotion. J Food Bioact. 2018; 3: 8–75. https://doi.org/10.31665/JFB.2018.3149
Gu C, Howell K, Dunshea FR, Suleria HA. LC-ESI-QTOF/MS characterisation of phenolic acids and flavonoids in polyphenol-rich fruits and vegetables and their potential antioxidant activities. Antioxidants. 2019; 8(9): 405. https://doi.org/10.3390/antiox8090405
Pandey B, Rajbhandari M. Estimation of total phenolic and flavonoid contents in some medicinal plants and their antioxidant activities. Nepal J Sci Technol. 2014; 15(1):53-60. https://doi.org/10.3126/njst.v15i1.12010
Mohammed S & Manan F A. Analysis of total phenolics, tannins and flavonoids from Moringa oleifera seed extract. J Chem Pharm Res. 2015; 7(1): 132-135. http://jocpr.com/vol7-iss1-2015/JCPR-2015-7-1-132-135
Makkar S, Blümmel M, Becker K. Formation of complexes between polyvinyl pyrrolidones or polyethylene glycols and tannins, and their implication in gas production and true digestibility in in vitro techniques. Br J Nutr. 1995; 73(6):897–913. https://doi.org/10.1079/BJN19950095
Salamatullah M, Hayat K, Arzoo S, Alzahrani A, Ahmed M A, Yehia H M, Althbiti MM. Boiling technique-based food processing effects on the bioactive and antimicrobial properties of basil and rosemary. Molecules. 2021; 26(23):7373. https://doi.org/10.3390/molecules26237373
Vuong V, Hirun S, Roach P D, Bowyer MC, Phillips P A, Scarlett CJ. Effect of extraction conditions on total phenolic compounds and antioxidant activities of Carica papaya leaf aqueous extracts. J Herb Med. 2013; 3(3):104–111. https://doi.org/10.1016/j.hermed.2013.04.004
Ghasemzadeh A, Jaafar H Z E, Rahmat A. Effects of solvent type on phenolics and flavonoids content and antioxidant activities in two varieties of young ginger (Zingiber officinale Roscoe) extracts. J Med Plants Res. 2011; 5(7):1147–1154.
Yeddes W, Majdi H, Gadhoumi H, Affes T. G, Mohamed S N, Aidi Wannes W, Saidani-Tounsi M. Optimizing ethanol extraction of rosemary leaves and their biological evaluations. J Explor Res Pharmacol. 2022; 7(2): 85–94. https://doi.org/10.14218/JERP.2022.00002
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